SUFU Antibody

SKU: orb1925587

Description

Purified Mouse Monoclonal Antibody (Mab)

Research Area

Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: IHC-P, KO/KD Validated, WB
• Dilution Range: WB - 1:2000, IHC-P - 1:25
• Reactivity: Human, Monkey, Mouse

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone No.: B3905EV758X485X41
• Immunogen: This SUFU antibody is generated from a mouse immunized with a recombinant protein of human SUFU.
• Target: SUFU {ECO:0000303|PubMed:12068298, ECO:0000312|HGNC:HGNC:16466}
• Molecular Weight: 53947 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Suppressor of fused homolog, SUFUH, SUFU

All lanes: Anti-SUFU Antibody at 1:2000 dilution. Lane 1: Hela whole cell lysate. Lane 2: COS-7 whole cell lysate. Lane 3: LNCap whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 54 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Staining SUFU in mouse embryo tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Quick Database Links

Gene Symbol

SUFU {ECO:0000303|PubMed:12068298, ECO:0000312|HGNC:HGNC:16466}

UniProt

Q9UMX1