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Streptavidin Antibody Biotin Conjugated

SKU: orb2652725

Description

Streptavidin Antibody Biotin Conjugated

Images & Validation

Tested ApplicationsDOT, ELISA
Dilution RangeELISA: 1:10,000 - 1:400,000
ReactivityBacteria
Application Notes
Biotin Conjugated Anti-Streptavidin Antibody has been tested by dot blot and is suitable to be assayed by ELISA for the detection of streptavidin in a standard ELISA using Peroxidase as a reporter. A working dilution of 1:10,000 to 1:400,000 of the reconstitution concentration is suggested for this product. Optimization of the concentration in immunoassays should be performed by the researcher. Reconstitution Buffer: Restore with deionized water (or equivalent). Reconstitution Volume: 1.0 mL

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenStreptavidin (Streptomyces avidinii)
PurityStreptavidin Antibody Biotin Conjugated was prepared from monospecific antiserum by delipidation, defibrination, salt fractionation and ion exchange chromatography. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum and Streptavidin. No reaction was observed against Avidin.
ConjugationBiotin

Storage & Handling

StorageStore vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/AppearanceLyophilized
Buffer/PreservativesPreservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free. 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Expiration Date12 months from date of receipt.
Hazard InformationThis product is for research use only and is not intended for therapeutic or diagnostic applications.
DisclaimerFor research use only

Alternative Names

rabbit anti-Streptavidin Antibody biotin Conjugation, biotin conjugated rabbit anti-Streptavidin antibody, Anti-Streptavidin BAC Antibody

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Protocol Information

ELISA
Enzyme-linked Immunosorbent Assay (EIA)
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DOT
Dot Blot
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