STRC Polyclonal Antibody

• Catalog Number: orb1417416
• Category: Antibodies
• Description: Rabbit polyclonal antibody to STRC.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, IHC-Fr, IHC-P, WB
• Reactivity: Rat
• Dilution range: WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC=1:100-500,IF=1:100-500,ELISA=1:5000-10000
• Conjugation: Unconjugated
• MW: 192967 Da
• UniProt ID: Q7RTU9
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Buffer/Preservatives: 0.01M TBS(pH7.4), 0.09% (W/V) sodium azide and 50% Glycerol
• Alternative names: Stereocilin, STRC
• Background: Essential to the formation of horizontal top connectors between outer hair cell stereocilia
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (STRC) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.