STRADA Antibody (C-term)

• Catalog Number: orb1927155
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB47810
• Tested applications: FC, IF, IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Dilution range: WB: 1:1000, IF: 1:25, WB: 1:1000, IHC-P: 1:100, FC: 1:25
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 48369 Da
• Target: This STRADA antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 303-346 amino acids from the C-terminal region of human STRADA.
• UniProt ID: Q7RTN6
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: STE20-related kinase adapter protein alpha, STRAD
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometric analysis of SH-SY5Y cells using STRADA Antibody (C-term) (green) compared to an isotype control of rabbit IgG (blue). Diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.

All lanes: Anti-STRADA Antibody (C-term) at 1:1000 dilution. Lane 1: 293 whole cell lysate. Lane 2: Molt-4 cell lysate. Lane 3: Mouse brain lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 48 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Fluorescent image of A549 cells stained with STRADA Antibody (C-term). Diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).

Immunohistochemical analysis on paraffin-embedded Human brain tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

All lanes: Anti-STRADA Antibody (C-term) at 1:1000 dilution. Lane 1: 293 whole cell lysate. Lane 2: HepG2 whole cell lysate. Lane 3: Molt-4 whole cell lysate. Lane 4: NIH/3T3 whole cell lysate. Lane 5: mouse brain lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 48 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.