STRADA Antibody (C-term)

SKU: orb1927155

Description

STRADA Antibody (C-term)

Research Area

Cancer Biology, Cell Biology, Metabolism Research

Images & Validation

• Tested Applications: FC, IF, IHC-P, WB
• Dilution Range: WB - 1:1000
• Reactivity: Human, Mouse, Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Molecular Weight: 48369 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.05% (V/V) Proclin 300. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

LYK5 {ECO:0000312|EMBL:AAP42280.1}, STRA

Flow cytometric analysis of SH-SY5Y cells using STRADA Antibody (C-term) (green) compared to an isotype control of rabbit IgG (blue). Diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.

All lanes: Anti-STRADA Antibody (C-term) at 1:1000 dilution. Lane 1: 293 whole cell lysate. Lane 2: Molt-4 cell lysate. Lane 3: Mouse brain lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 48 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Fluorescent image of A549 cells stained with STRADA Antibody (C-term). Diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).

Immunohistochemical analysis on paraffin-embedded Human brain tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

All lanes: Anti-STRADA Antibody (C-term) at 1:1000 dilution. Lane 1: 293 whole cell lysate. Lane 2: HepG2 whole cell lysate. Lane 3: Molt-4 whole cell lysate. Lane 4: NIH/3T3 whole cell lysate. Lane 5: mouse brain lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 48 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

UniProt

Q7RTN6