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Stomatin Antibody

SKU: orb1248486

Description

Stomatin Antibody

Research Area

Immunology & Inflammation

Images & Validation

Tested ApplicationsELISA, IHC, WB
ReactivityHuman
Application Notes
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 30kDa band observed in Human Erythrocytes, Spleen, Bone Marrow and Lung lysates (calculated MW of 31.7kDa according to NP_004090.4). Recommended concentration: 0.1-0.3µg/mL.Immunohistochemistry:In paraffin embedded Human Liver shows membrane staining of hepatocytes and of sinusoidal lining cells. Recommended concentration, 5-10µg/mL.

Key Properties

Antibody TypePrimary Antibody
HostGoat
ClonalityPolyclonal
ImmunogenThe immunogen for this antibody is: EKRHTRDSEAQR-C
TargetStomatin
Molecular WeightApprox 30kDa
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration500 ug/mL
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

STOM, stomatin, BND7, EPB7, EPB72, erythrocyte band 7 integral membrane protein, erythrocyte membrane protein band 7.2 (stomatin), protein 7.2b

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Stomatin Antibody

orb1248486 (0.1 ug/ml) staining of Human Lung lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Stomatin Antibody

orb1248486 (5 ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

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Datasheet
Product Information
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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Stomatin Antibody (orb1248486)

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0.1 mg
$ 920.00
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