STK35 Antibody

• Catalog Number: orb1246898
• Category: Antibodies
• Description: STK35 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IF, IHC, WB
• Predicted Reactivity: Bovine, Canine, Rat
• Reactivity: Human, Mouse, Porcine
• Immunogen: The immunogen for this antibody is: ELETRMDQVTCAA
• Antibody Type: Primary Antibody
• Concentration: 500 ug/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: STK35
• UniProt ID: Q8TDR2
• NCBI: NP_543026.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: STK35, Clik1, bA550O8.2, bA550O8.2 CLIK1, serine/t
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 55kDa band observed in Human Breast cancer and in Mouse and Pig Testis lysates, and approx. 58kDa in nuclear lysates of cell line HEK293 (calculated MW of 58.1kDa according to Human NP_543026.2, 59.5kDa according to Mouse NP_899085.3 and 59.2kDa according to XP_005672850.1 Pig XP_005672850.1). Recommended concentration: 1-3ug/ml. Primary incubation 1 hour at room temperature. This product has been successfully used in WB on Human: Goyal P et al, PLoS One. 2009 Sep 16;4(9):e6981. PMID: 19756140.Immunohistochemistry:In paraffin embedded Human Testis shows granular cytoplasmic staining of Leydig cells. Recommended concentration: 10ug/ml.Immunofluorescence: Strong expression of the protein seen in the nuclei of U2OS cells. Recommended concentration: 10ug/ml. Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1246898 (2 ug/ml) staining of nuclear HEK293 (A) and (1 ug/ml) Human Breast cancer (B) lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1246898 (10 ug/ml) staining of paraffin embedded Human Testis. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.

orb1246898 (1 ug/ml) staining of Mouse (A) and (2 ug/ml) Pig (B) Testes lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1246898 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue).

orb1246898 Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line).