Steroidogenic Factor 1 Antibody / SF-1 / NR5A1

• Catalog Number: orb1716256
• Category: Antibodies
• Description: Steroidogenic Factor 1 (SF-1) is considered an orphan nuclear receptor that belongs to subfamily 5. It was found to be a regulator of steroidogenic enzyme gene expression. Oxysterols are suggested as its ligands. It is expressed in all steroidogenic tissues, including the adrenal cortex, testicular Sertoli cells, and Leydig cells, ovarian theca, hypothalamus, and anterior pituitary. SF-1 plays an important role in adrenal and gonadal development. SF-1 is highly valuable marker to determine the adrenocortical origin of an adrenal mass.
• Species/Host: Mouse
• Clonality: Recombinant
• Clone Number: rNR5A1/4369
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: A portion of amino acids 220-461 from the human protein was used as the immunogen for the recombinant Steroidogenic Factor 1 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml
• Conjugation: Unconjugated
• Formula: 1 mg/ml in 1X PBS; BSA free, sodium azide free
• Hazard Information: This recombinant Steroidogenic Factor 1 antibody is available for research use only.
• UniProt ID: Q13285
• Storage: Aliquot the recombinant Steroidogenic Factor 1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Note: For research use only
• Application notes: Optimal dilution of the recombinant Steroidogenic Factor 1 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human renal oncocytoma with recombinant Steroidogenic Factor 1 antibody (clone rNR5A1/4369). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free recombinant Steroidogenic Factor 1 antibody (clone rNR5A1/4369) as confirmation of integrity and purity.