STEP / PTPN5 Antibody (N-Term)

• Catalog Number: orb1928508
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB00604
• Tested applications: IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, WB: 1:1000, IHC-P: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 63538 Da
• Target: This STEP / PTPN5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 168-198 amino acids from the N-terminal region of human STEP / PTPN5.
• UniProt ID: P54829
• NCBI: NP_001265167.1, NP_001265165.1, NP_116170.3, NP_001035059.1, NP_008837.1, NP_001265168.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Tyrosine-protein phosphatase non-receptor type 5,
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of hSTEP-Q155 in HepG2 cell line lysates (35 ug/lane). STEP (arrow) was detected using the purified Pab.

Western blot analysis of hSTEP-Q155 in mouse brain tissue lysates (35 ug/lane). STEP (arrow) was detected using the purified Pab

Formalin-fixed and paraffin-embedded human brain tissue reacted with STEP antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.