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STEAP1/STEAP Antibody

SKU: orb1537960

Description

STEAP1/STEAP Antibody

Images & Validation

Tested ApplicationsELISA, ICC, IF, IHC, IHC-P, WB
Dilution rangeELISA, ICC, IF, IHC, IHC-P (10 µg/ml), WB (1:500 - 1:1000)
ReactivityHuman
Application Notes
Further information: Immunohistochemistry: LS-B3003 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3003 was determined to be 10 ug/ml.

Key Properties

HostMouse
ClonalityMonoclonal
IsotypeIgG2b,k
Clone No.J2D2
ImmunogenRecombinant human STEAP1 (1-70aa) purified from E. coli. NCBI Accession No: NP_036581.
TargetSTEAP1 / STEAP
PurificationProtein G purified

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesPBS, pH 7.4, 0.02% Sodium Azide, 10% Glycerol. PBS, pH 7.4, 0.02% Sodium Azide, 10% Glycerol
Concentration1 mg/ml
DisclaimerFor research use only

Alternative Names

STEAP1, Metalloreductase STEAP1, PRSS24, STEAP

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STEAP1/STEAP Antibody

Immunofluorescence staining of human LNCap cell colony with monoclonal antihuman STEAP1 antibody

STEAP1/STEAP Antibody

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
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IHC-P
Immunohistochemistry Paraffin
View Protocol
IF
Immunofluorescence
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ICC
Immunocytochemistry
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ELISA
Enzyme-linked Immunosorbent Assay (EIA)
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STEAP1/STEAP Antibody (orb1537960)

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50 μl
$ 660.00
DispatchUsually dispatched within 2-3 weeks
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