You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb1537960 |
---|---|
Category | Antibodies |
Description | STEAP1/STEAP Antibody |
Target | STEAP1 / STEAP |
Clonality | Monoclonal |
Species/Host | Mouse |
Isotype | IgG2b,k |
Conjugation | Unconjugated |
Reactivity | Human |
Concentration | 1 mg/ml |
Buffer/Preservatives | PBS, pH 7.4, 0.02% Sodium Azide, 10% Glycerol. PBS, pH 7.4, 0.02% Sodium Azide, 10% Glycerol |
Purification | Protein G purified |
Immunogen | Recombinant human STEAP1 (1-70aa) purified from E. coli. NCBI Accession No: NP_036581. |
Tested applications | ELISA, ICC, IF, IHC, IHC-P, WB |
Dilution range | ELISA, ICC, IF, IHC, IHC-P (10 µg/ml), WB (1:500 - 1:1000) |
Application notes | Further information: Immunohistochemistry: LS-B3003 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3003 was determined to be 10 ug/ml. |
Clone Number | J2D2 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | STEAP1, Metalloreductase STEAP1, PRSS24, STEAP |
Note | For research use only |
Immunofluorescence staining of human LNCap cell colony with monoclonal antihuman STEAP1 antibody