STEA2 Antibody (Center)

• Catalog Number: orb1930441
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB26961
• Tested applications: FC, IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Dilution range: WB: 1:500-1:1000, WB: 1:500, IHC-P: 1:50~100, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 56056 Da
• Target: This STEAP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 229-258 amino acids of human STEAP2.
• UniProt ID: Q8NFT2
• NCBI: NP_001231873.1, NP_694544.2, NP_001035755.1, NP_001035756.1, NP_001231875.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Metalloreductase STEAP2, 1161-, Prostate cancer-as
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

STEAP2 Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

STEAP2 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the STEAP2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

Western blot analysis of extracts from 293 (lanes 1 and 2), HepG2 (lanes 3 and 4) cell line and mouse spleen (lanes 5 and 6) tissue lysate: 1, 3, 5. using STEAP2 Antibody (Center), (1:500). 2, 4, 6. using STEAP2 Antibody (Center), preincubated with the control peptide antigen.

Western blot analysis of lysates from 293, HepG2 cell line, mouse spleen tissue lysate (from left to right), using STEAP2 Antibody (Center). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20 ug per lane.