STEA2 Antibody (Center)

SKU: orb1930441

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Cancer Biology

Images & Validation

• Tested Applications: FC, IHC-P, WB
• Dilution Range: WB-1:500-1:1000, FC-1:10~50, IHC-P-1:50~100
• Reactivity: Human, Mouse

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB26961
• Immunogen: This STEAP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 229-258 amino acids of human STEAP2. Antigen Region: 229-258 aa.
• Target: STEAP2
• Molecular Weight: 56056 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Metalloreductase STEAP2, 1161-, Prostate cancer-associated protein 1, Protein up-regulated in metastatic prostate cancer, PUMPCn, Six-transmembrane epithelial antigen of prostate 2, SixTransMembrane protein of prostate 1, STEAP2, PCANAP1, STAMP1

Western blot analysis of extracts from 293 (lanes 1 and 2), HepG2 (lanes 3 and 4) cell line and mouse spleen (lanes 5 and 6) tissue lysate: 1, 3, 5. using STEAP2 Antibody (Center), (1:500). 2, 4, 6. using STEAP2 Antibody (Center), preincubated with the control peptide antigen.

Western blot analysis of lysates from 293, HepG2 cell line, mouse spleen tissue lysate (from left to right), using STEAP2 Antibody (Center). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20 ug per lane.

STEAP2 Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

STEAP2 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the STEAP2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

Quick Database Links

Gene Symbol

STEAP2

UniProt

Q8NFT2

RefSeq (Protein):NP_694544.2, NP_001035756.1, NP_001035755.1, NP_001231875.1, NP_001231873.1