STAT3 phospho Y705 Antibody

SKU: orb345698

Description

STAT3 (phospho-Y705) antibody

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:10,000 - 1:30,000, WB: 1 µg/mL
• Reactivity: Human
• Application Notes:
  STAT3 pY705 antibody has been tested for use in ELISA, IHC, and Western Blot. Expect a band approximately 88.1 kDa in size corresponding to phosphorylated STAT3 protein by western blotting in the appropriate stimulated tissue or cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: This affinity purified Stat3 antibody was prepared by repeated immunizations with a phosphorylated synthetic peptide corresponding to the region of amino acids surrounding tyrosine 705 of human Stat3.
• Purity: Anti-STAT3 antibody is an affinity-purified antibody and is directed against the phosphorylated form of human STAT3 protein at the Y705. Anti-Stat3 antibody was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide. Anti-Stat3 antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated Stat3 protein is minimal by ELISA and western blot. A BLAST analysis was used to suggest cross reactivity with Stat3 antibodies from human, mouse, rat, and pig based on 100%sequence homology with the immunogen. Reactivity against homologues from other sources is not known.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at-20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-STAT3 pY705 antibody, Signal transducer and activator of transcription 3, Acute-phase response factor, APRF, STAT3 phospho Y705, STAT3 (pTry705)

Immunohistochemistry of Rabbit anti STAT3 pY705 Antibody. Tissue: Human Colon at 40X in colon tissue at pH9. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: STAT3 pY705 antibody at 10 µg/ml for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10000 for 45 min at RT. Localization: STAT3 phospho Y705 is nuclear and cytoplasmic. Staining: STAT3 (pTyr705) as precipitated red signal with hematoxylin purple nuclear counterstain.

Western Blot of Rabbit anti-Stat3 pY705 antibody. Lane 1: Jurkat lysate. Lane 2: Jurkat lysate IFN-α treatment. Load: 35 µg per lane. Primary antibody: STAT3 pY705 antibody at 1:1000 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO (p/n orb348624) overnight at 4°C. Predicted/Observed size: 88 kDa, ~90kDa for STAT3py705. Other band(s): STAT 3 pY705 splice variants and isoforms.

Quick Database Links

UniProt

P40763

RefSeq (Protein):NP_644805.1