STAT3 Phospho (Tyr705) Mouse Monoclonal Antibody

SKU: orb1735066

Description

STAT3 Phospho (Tyr705) Mouse Monoclonal Antibody

Images & Validation

• Tested Applications: WB
• Dilution Range: Western Blot 1:500-1:1000, Immunofluorescence 1:200–1:400
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Immunogen: Synthetic peptide corresponding to residues surrounding Tyr705 of human STAT3
• Target: STAT3 Phospho (Tyr705)
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

orb1735066 anti-Phospho STAT3 (Tyr705) monoclonal antibody at 1/500 dilution, Total cell lysates (15 µg total protein) from A431 cells serum starved and untreated (-) or stimulated with 100 ng/mL hEGF for 5 minutes. This blot was produced using a 7.5% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1735066 overnight at 4°C.

Serum starved A431 cells were untreated (panel A) or stimulated with 100 ng/mL hEGF for 5 minutes (panel B). Cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1735066 anti-Phospho STAT3 (Tyr705) monoclonal antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Quick Database Links

Gene Symbol

STAT3 Phospho (Tyr705)