STAT3 Antibody

SKU: orb1249600

Description

STAT3 Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, FC, IF, IHC
• Reactivity: Mouse
• Predicted Reactivity: Bovine, Canine, Human, Porcine, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:8000.Immunohistochemistry:In paraffin embedded Mouse Thymus shows preferential staining of the Medulla over the Cortex. Recommended concentration: 4-6µg/mL.Immunofluorescence:Strong expression of the protein seen in the cytoplasm of HeLa and A431 cells. Recommended concentration: 10µg/mL. Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: DMELTSECATSPM
• Target: STAT3
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

STAT3, signal transducer and activator of transcription 3 (acute-phase response factor), APRF, DNA-binding protein APRF, acute-phase response factor, FLJ20882, MGC16063, HIES

orb1249600 (4 ug/ml) staining of paraffin embedded Mouse Thymus. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.

Quick Database Links

Gene Symbol

STAT3

UniProt

P40763

RefSeq (Protein):NP_644805.1, NP_003141.2