STAT1 (phospho-Y701) antibody

• Catalog Number: orb344640
• Category: Antibodies
• Description: STAT1 (phospho-Y701) antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: This Protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding Y701 in the mouse STAT1 protein.
• Concentration: 2.3 mg/mL
• Dilution range: ELISA: 1:25,000, WB: 1:500 - 1:2,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: This product was Protein A purified from monospecific antiserum. This antibody is specific for mouse Stat1 protein phosphorylated at Y701. A BLAST analysis was used to suggest cross-reactivity with Stat1 from mouse and rat based on 100% homology with the immunizing sequence. Partial reactivity is expected against Stat1 from human sources as 90% homology is noted. Cross-reactivity with Stat1 from other sources has not been determined.
• Conjugation: Unconjugated
• UniProt ID: Q99K94
• NCBI: 114326482
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-Stat1 pY701 Antibody, DKFZp686B04100 a
• Note: For research use only
• Application notes: This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 91 kDa in size corresponding to phosphorylated Stat1 protein by western blotting in the appropriate cell lysate or extract. This phospho-specific polyclonal antibody reacts with mouse Stat1 pY701 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of K562 cells lysates using STAT1 (phospho-Y701) antibody