Stat1 (phospho Tyr701) rabbit pAb

SKU: orb764279

Description

Rabbit polyclonal antibody to Stat1 (phospho-Tyr701)

Images & Validation

• Tested Applications: ELISA, IF, IHC-P, IP, WB
• Dilution range: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. ELISA: 1/10000. Not yet tested in other applications.
• Reactivity: Human, Monkey, Mouse, Rat

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human STAT1 around the phosphorylation site of Tyr701. AA range:668-717
• Molecular Weight: 87
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Anti-STAT1 antibody, anti-Signal transducer and activator of transcription 1-alpha/beta antibody, anti-Transcription factor ISGF-3 components p91/p84 antibody

Immunofluorescence analysis of Hela cell. 1, Stat1 (phospho Tyr701) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). HER2 Monoclonal Antibody (11H9) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min).

Western blot analysis of lysates from 1) MOUSE-BRAIN, 2) SY-SY5X , 3) COS7 cells, was diluted at 1:10000, 37° 1hour. antibody was diluted at 1:5000 as loading control, 4°C over night, secondary antibody was diluted at 1:10000, 37° 1hour.

Immunofluorescence analysis of rat-spleen tissue. 1, Stat1 (phospho Tyr701) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A + B.

Immunofluorescence analysis of rat-spleen tissue. 1, Stat1 (phospho Tyr701) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A + B.