STAT1 Antibody

• Catalog Number: orb1261079
• Category: Antibodies
• Description: STAT1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 650-750 of human STAT1 (NP_009330.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:20 - 1:50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 91kDa
• Target: STAT1
• UniProt ID: P42224
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: STAT1 alpha Antibody: CANDF7, ISGF-3, STAT91
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:20 - 1:50
• Expiration Date: 12 months from date of receipt.

Immunoprecipitation analysis of 100 ug extracts of HeLa cells using 3 ug STAT1 antibody (orb1261079). Western blot was performed from the immunoprecipitate using STAT1 antibody (orb1261079) at a dilution of 1:1000.

Immunofluorescence analysis of U2OS cells using STAT1 antibody (orb1261079) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunohistochemistry of paraffin-embedded human gastric cancer using STAT1 antibody (orb1261079) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human mammary cancer using STAT1 antibody (orb1261079) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of HeLa cells, using STAT1 antibody (orb1261079) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.