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STAT1 alpha Antibody

Catalog Number: orb1240037

DispatchUsually dispatched within 5-10 working days
$ 670.00
Catalog Numberorb1240037
CategoryAntibodies
DescriptionSTAT1 alpha Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsELISA, ICC, IF, IHC-P, IP, WB
Predicted ReactivityPorcine
ReactivityHuman, Mouse, Rat
IsotypeIgG
ImmunogenAnti-STAT1 alpha antibody (orb1240037) was raised against a peptide corresponding to 38 amino acids near the carboxy terminus of human STAT1 alpha. The immunogen is located within the last 50 amino acids of STAT1 alpha.
Antibody TypePrimary Antibody
Concentration1 mg/ml
Form/AppearanceLiquid
ConjugationUnconjugated
MWPredicted: 87kDObserved: 87 kD
TargetSTAT1
UniProt IDP42224
NCBINP_009330
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSTAT1 alpha Antibody is supplied in PBS containing 0.02% sodium azide.
Alternative namesSTAT1 alpha Antibody: CANDF7, ISGF-3, STAT91
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NoteFor research use only
Expiration Date12 months from date of receipt.
STAT1 alpha Antibody

Western Blot Validation in Human and Mouse Cell Lines of (H) Hela, (J) Jurkat, (A) A431, (K) K562 and (N) 3T3/NIH. Loading: 15 µg of lysates per lane. Antibodies: STAT1 alpha orb1240037 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

STAT1 alpha Antibody

Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: STAT1 alpha orb1240037 (1 µg/mL), competitor antibody (2 µg/mL), and beta-actin (1.5 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

STAT1 alpha Antibody

Western Blot Validation in Mouse tissues. Loading: 15 µg of lysates per lane. Antibodies: STAT1 alpha orb1240037 (0.5 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

STAT1 alpha Antibody

Western Blot Validation in Rat Cell Line. Loading: 15 µg of lysates per lane. Antibodies: STAT1 alpha orb1240037 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

STAT1 alpha Antibody

Immunofluorescence Validation of STAT1 alpha in Human Colon Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed Human Colon Tissue labeling STAT1 alpha with orb1240037 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

STAT1 alpha Antibody

Immunohistochemistry Validation of STAT1 alpha in Human Colon Tissue. Immunohistochemical analysis of paraffin-embedded Human Colon Tissue using anti-STAT1 alpha antibody (orb1240037) at 2.5 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

STAT1 alpha Antibody

Immunocytochemistry Validation of STAT1 alpha in Human HeLa Cells. Immunocytochemical analysis of HeLa cells using anti-STAT1 alpha antibody (orb1240037) at 10 µg/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

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