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STAT1 alpha Antibody

Catalog Number: orb1240037

DispatchUsually dispatched within 5-10 working days
$ 240.00
Catalog Numberorb1240037
CategoryAntibodies
DescriptionSTAT1 alpha Antibody
TargetSTAT1
ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ReactivityHuman, Mouse, Rat
Predicted ReactivityPorcine
Form/AppearanceLiquid
Concentration1 mg/ml
Buffer/PreservativesSTAT1 alpha Antibody is supplied in PBS containing 0.02% sodium azide.
ImmunogenAnti-STAT1 alpha antibody (orb1240037) was raised against a peptide corresponding to 38 amino acids near the carboxy terminus of human STAT1 alpha. The immunogen is located within the last 50 amino acids of STAT1 alpha.
UniProt IDP42224
MWPredicted: 87kDObserved: 87 kD
Tested applicationsELISA, ICC, IF, IHC-P, IP, WB
Antibody TypePrimary Antibody
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesSTAT1 alpha Antibody: CANDF7, ISGF-3, STAT91
NoteFor research use only
NCBINP_009330
STAT1 alpha Antibody

Western Blot Validation in Human and Mouse Cell Lines of (H) Hela, (J) Jurkat, (A) A431, (K) K562 and (N) 3T3/NIH. Loading: 15 µg of lysates per lane. Antibodies: STAT1 alpha orb1240037 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

STAT1 alpha Antibody

Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: STAT1 alpha orb1240037 (1 µg/mL), competitor antibody (2 µg/mL), and beta-actin (1.5 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

STAT1 alpha Antibody

Western Blot Validation in Mouse tissues. Loading: 15 µg of lysates per lane. Antibodies: STAT1 alpha orb1240037 (0.5 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

STAT1 alpha Antibody

Western Blot Validation in Rat Cell Line. Loading: 15 µg of lysates per lane. Antibodies: STAT1 alpha orb1240037 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

STAT1 alpha Antibody

Immunofluorescence Validation of STAT1 alpha in Human Colon Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed Human Colon Tissue labeling STAT1 alpha with orb1240037 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

STAT1 alpha Antibody

Immunohistochemistry Validation of STAT1 alpha in Human Colon Tissue. Immunohistochemical analysis of paraffin-embedded Human Colon Tissue using anti-STAT1 alpha antibody (orb1240037) at 2.5 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

STAT1 alpha Antibody

Immunocytochemistry Validation of STAT1 alpha in Human HeLa Cells. Immunocytochemical analysis of HeLa cells using anti-STAT1 alpha antibody (orb1240037) at 10 µg/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

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