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Catalog Number | orb1240037 |
---|---|
Category | Antibodies |
Description | STAT1 alpha Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, ICC, IF, IHC-P, IP, WB |
Predicted Reactivity | Porcine |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | Anti-STAT1 alpha antibody (orb1240037) was raised against a peptide corresponding to 38 amino acids near the carboxy terminus of human STAT1 alpha. The immunogen is located within the last 50 amino acids of STAT1 alpha. |
Antibody Type | Primary Antibody |
Concentration | 1 mg/ml |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Predicted: 87kDObserved: 87 kD |
Target | STAT1 |
UniProt ID | P42224 |
NCBI | NP_009330 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | STAT1 alpha Antibody is supplied in PBS containing 0.02% sodium azide. |
Alternative names | STAT1 alpha Antibody: CANDF7, ISGF-3, STAT91 Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western Blot Validation in Human and Mouse Cell Lines of (H) Hela, (J) Jurkat, (A) A431, (K) K562 and (N) 3T3/NIH. Loading: 15 µg of lysates per lane. Antibodies: STAT1 alpha orb1240037 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: STAT1 alpha orb1240037 (1 µg/mL), competitor antibody (2 µg/mL), and beta-actin (1.5 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation in Mouse tissues. Loading: 15 µg of lysates per lane. Antibodies: STAT1 alpha orb1240037 (0.5 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation in Rat Cell Line. Loading: 15 µg of lysates per lane. Antibodies: STAT1 alpha orb1240037 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Immunofluorescence Validation of STAT1 alpha in Human Colon Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed Human Colon Tissue labeling STAT1 alpha with orb1240037 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
Immunohistochemistry Validation of STAT1 alpha in Human Colon Tissue. Immunohistochemical analysis of paraffin-embedded Human Colon Tissue using anti-STAT1 alpha antibody (orb1240037) at 2.5 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunocytochemistry Validation of STAT1 alpha in Human HeLa Cells. Immunocytochemical analysis of HeLa cells using anti-STAT1 alpha antibody (orb1240037) at 10 µg/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
IF, IHC-Fr, IHC-P, WB | |
Mouse, Rat | |
Human, Mouse, Rat | |
Rabbit | |
Recombinant | |
Unconjugated |
FC, ICC, IF, IHC-Fr, IHC-P, WB | |
Bovine, Canine, Equine, Porcine, Sheep | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, IHC-Fr, IHC-P, WB | |
Human | |
Human | |
Rabbit | |
Recombinant | |
Unconjugated |
IF, IHC-P, IP, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, IHC, WB | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |