Stanniocalcin-2 (STC2) Antibody (N-term)

• Catalog Number: orb1935808
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB12352
• Tested applications: IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, WB: 1:1000, WB: 1:1000, WB: 1:500, IHC-P: 1:50~100
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 33249 Da
• Target: This Stanniocalcin-2 (STC2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 16-46 amino acids from the N-terminal region of human Stanniocalcin-2 (STC2).
• UniProt ID: O76061
• NCBI: NP_003705.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Stanniocalcin-2, STC-2, Stanniocalcin-related prot
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of anti-STC2 Antibody (N-term) in mouse kidney tissue lysates (35 ug/lane). STC2 (arrow) was detected using the purified polyclonal antibody.

All lanes: Anti-Stanniocalcin-2 (STC2) Antibody (N-term) at 1:1000 dilution. Lane 1: A431 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Mouse IgG/A/M (H/L), Peroxidase conjugated at 1/2000 dilution.Observed band size: 33kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-Stanniocalcin-2 (STC2) Antibody (N-term) at 1:500 dilution. Lane 1: mouse skeletal muscle tissue lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Mouse IgG/A/M (H/L), Peroxidase conjugated at 1/2000 dilution.Observed band size: 32kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Stanniocalcin-2 (STC2) Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of Stanniocalcin-2 (STC2) Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

All lanes: Anti-Stanniocalcin-2 (STC2) Antibody (N-term) at 1:500 dilution. Lane 1: A431 whole cell lysate. Lane 2: U-2OS whole cell lysate. Lane 3: U-251 MG whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 33 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.