Stanniocalcin-2 (STC2) Antibody (N-term)

SKU: orb1935808

Description

Stanniocalcin-2 (STC2) Antibody (N-term)

Research Area

Infectious Disease & Virology, Signal Transduction

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB - 1:500
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Molecular Weight: 33249 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Western blot analysis of anti-STC2 Antibody (N-term) in mouse kidney tissue lysates (35 ug/lane). STC2 (arrow) was detected using the purified polyclonal antibody.

All lanes: Anti-Stanniocalcin-2 (STC2) Antibody (N-term) at 1:1000 dilution. Lane 1: A431 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Mouse IgG/A/M (H/L), Peroxidase conjugated at 1/2000 dilution.Observed band size: 33kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-Stanniocalcin-2 (STC2) Antibody (N-term) at 1:500 dilution. Lane 1: mouse skeletal muscle tissue lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Mouse IgG/A/M (H/L), Peroxidase conjugated at 1/2000 dilution.Observed band size: 32kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Stanniocalcin-2 (STC2) Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of Stanniocalcin-2 (STC2) Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

All lanes: Anti-Stanniocalcin-2 (STC2) Antibody (N-term) at 1:500 dilution. Lane 1: A431 whole cell lysate. Lane 2: U-2OS whole cell lysate. Lane 3: U-251 MG whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 33 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

UniProt

O76061