ST2 Antibody

• Catalog Number: orb1240035
• Category: Antibodies
• Description: ST2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, IHC-P, WB
• Predicted Reactivity: Bovine, Equine
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: ST2 antibody was raised against a synthetic peptide corresponding to 16 amino acids at the amino-terminus of mouse ST2.This peptide is common to all three known ST2 isoforms.The immunogen is located within amino acids 40 - 90 of ST2.
• Antibody Type: Primary Antibody
• Concentration: 1 mg/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 63/37 kD Observed: 63/37 kD
• Target: Il1rl1
• UniProt ID: P14719
• NCBI: BAA02854
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: ST2 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: ST2 Antibody: T1, St2, DER4, Ly84, ST2L, Fit-1, T1
• Note: For research use only
• Application notes: WB: 1-2 μg/mL; IHC: 1-5 μg/mL; IF: 5-20 μg/mL. Antibody validated: Western Blot in human, mouse and rat samples; Immunocytochemistry and Immunofluorescence in human, mouse and rat samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Immunoflouorescence Validation of ST2 in HeLa Cells. Immunofluorescent analysis of PFA-fixed HeLa cells labeling ST2 with orb1240035 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and DAPI staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.

WB Validation in Human, Mouse, and Rat Cell Lines. Loading: 15 µg of lysate per lane Antibodies: ST2 orb1240035, 1 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in 293 Cells. Antibodies: ST2 orb1240035, 1 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane A: In the absence of blocking peptide Lane B: In the presence of blocking peptide.

Western Blot Validation in Mouse Heart. Loading: 15 µg of lysate Antibodies: ST2 orb1240035, 1 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunofluorescence Validation of ST2 in Human Lung. Immunofluorescent analysis of PFA-fixed human lung tissue labeling ST2 with orb1240035 at 10 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and DAPI staining (blue).

Immunofluorescence Validation of ST2 in Human Lung Cancer Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed human lung cancer tissue labeling ST2 with orb1240035 at 5 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) DAPI staining (blue).

Immunofluorescence Validation of ST2 in Mouse Kidney. Immunofluorescent analysis of PFA-fixed mouse kidney tissue labeling ST2 with orb1240035 at 10 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and DAPI staining (blue).

Immunohistochemistry Validation of ST2 in Rat Kidney. Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-ST2 antibody (orb1240035) at 5 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.