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Catalog Number | orb1248063 |
---|---|
Category | Antibodies |
Description | SRRT / ARS2 Antibody |
Target | SRRT / ARS2 |
Clonality | Polyclonal |
Conjugation | Unconjugated |
Reactivity | Human, Mouse, Rat |
Predicted Reactivity | Bovine, Canine, Porcine |
Form/Appearance | Liquid |
Concentration | 500 ug/mL |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Immunogen | The immunogen for this antibody is: C-DPRAIVEYRDLDAPD |
UniProt ID | Q9BXP5 |
MW | Approx 140kDa |
Tested applications | ELISA, IHC, WB |
Application notes | Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 140kDa band observed in lysates of cell line Daudi and of Mouse Spleen, and approx 150kDa band observed in lysates of Rat Testis (calculated MW of 101kDa according to Human NP_056992.4, 100kDa according to Mouse NP_113582.1 and 88.4kDa according to Rat XP_006221478.1). The observed molecular weight is explained by the acetylation of the protein and it corresponds to earlier findings with different antibodies from other commercial sources. An additional band of unknown identity was also consistently observed at 37kDa in Mouse Spleen. This band was successfully blocked by incubation with the immunizing peptide. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Uterus. Recommended concentration: 5ug/ml. |
Antibody Type | Primary Antibody |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | SRRT, serrate RNA effector molecule homolog (Arabi Read more... |
Note | For research use only |
NCBI | NP_001122326.1, NP_001122324.1, NP_056992.4, NP_001122325.1 |
orb1248063 (0.1 ug/ml) staining of Daudi (A) and Mouse Spleen (B) lysates (35 ug protein in RIPA buffer). Detected by chemiluminescence.
orb1248063 (0.1 ug/ml) staining of Rat Testis lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
orb1248063 (5 ug/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
ELISA, IHC, WB | |
Bovine, Canine, Human, Mouse, Porcine, Rat | |
Goat | |
Polyclonal | |
Unconjugated |