SRF Antibody

SKU: orb1248931

Description

SRF Antibody

Research Area

Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: ELISA, WB
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 67kDa band observed in primary cultured Human Lung fibroblasts lysates after 24 h treatment with 1ng/ml TGF-bl (calculated MW of 51.6kDa according to NP_003122.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Davis FJ et al, Am J Physiol Heart Circ Physiol. 2002 Apr;282(4):H1521-33.; PMID: 11893590). Recommended concentration: 1-3µg/mL.

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Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-QASPSRDSSTDLTQ
• Target: SRF
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

SRF, serum response factor (c-fos serum response element-binding transcription factor), MCM1, OTTHUMP00000039820

Quick Database Links

Gene Symbol

SRF

UniProt

P11831

RefSeq (Protein):NP_003122.1