SRC Antibody (Y419)

• Catalog Number: orb1928408
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB21492
• Tested applications: IF, IHC-P, WB
• Predicted Reactivity: Mouse, Other, Porcine, Rat, Zebrafish
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: IF: 1:10~50, WB: 1:1000, WB: 1:1000, IHC-P: 1:50~100
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 59835 Da
• Target: This SRC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 397-424 amino acids from human SRC.
• UniProt ID: P12931
• NCBI: NP_938033.1, NP_005408.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Proto-oncogene tyrosine-protein kinase Src, Proto-
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of SRC Antibody (Y419) in HepG2 cell line lysates (35 ug/lane). SRC (arrow) was detected using the purified Pab.

Western blot analysis of lysate from A375 cell line, using SRC Antibody (Y419). Diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20 ug.

Confocal immunofluorescent analysis of SRC Antibody (Y419) with A375 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).

Formalin-fixed and paraffin-embedded human hepatocarcinoma with SRC Antibody (Y419), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.