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sRANK Ligand Antibody (biotin)
Catalog Number: orb1272305
| Catalog Number | orb1272305 |
|---|---|
| Category | Antibodies |
| Description | sRANK Ligand Antibody (biotin) |
| Species/Host | Goat |
| Clonality | Polyclonal |
| Tested applications | ELISA, WB |
| Reactivity | Human |
| Immunogen | Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hsRANKL (Human sRANK-Ligand). |
| Antibody Type | Primary Antibody |
| Concentration | batch dependent |
| Form/Appearance | Lyophilized |
| Conjugation | Biotin |
| Target | TNFSF11 |
| UniProt ID | O14788 |
| NCBI | O14788 |
| Storage | sRANK Ligand antibody is stable for at least 2 years from date of receipt at -20˚°C. The reconstituted antibody is stable for at least two weeks at 2-8˚°C. Frozen aliquots are stable for at least 6 months when stored at -20˚°C. Avoid repeated freeze-thaw cycles. |
| Alternative names | ODF, OPGL, sOdf, CD254, OPTB2, RANKL, TRANCE, hRAN Read more... |
| Note | For research use only |
| Application notes | ELISA:Sandwich:To detect hsRANKL by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human sRANKL (XP-5274) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hsRANKL. Western Blot:To detect hsRANKL by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsRANKL is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
| Expiration Date | 12 months from date of receipt. |
To detect hsRANKL by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 – 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Anti-Human sRANKL (orb1272306) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hsRANKL.
To detect hsRANKL by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsRANKL is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect hsRANKL by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsRANKL is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
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