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Description
Research Area
Cell Biology
Images & Validation
−
| Tested Applications | FC, ICC, IF, IHC-Fr, IHC-P, IP, WB |
|---|---|
| Dilution Range | WB=1:500-2000, IHC-P=1:50-200, IHC-F=1:50-200, IF=1:50-200, Flow-Cyt=1:50-100, ICC/IF=1:50-200, IP=1:20-50 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Recombinant |
| Isotype | IgG |
| Clone No. | B6D0 |
| Immunogen | A synthesized peptide derived from human Sequestosome 1 (2-440aa) |
| Target | SQSTM1 |
| Molecular Weight | 60 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−A170; DMRV; EBIAP; FTDALS3; NADGP; OSIL; PDB3; ZIP3; p60; p62; p62B; OSF-6; Osi; STAP; STONE14; ZIP; SQSTM_HUMAN; SQSTM1; EBI3-associated protein of 60 kDa (EBIAP | p60); Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa; Ubiquitin-binding protein p62 (p62); ORCA; SQSTM_MOUSE; Ubiquitin-binding protein p62; SQSTM_RAT; Protein kinase C-zeta-interacting protein (PKC-zeta-interacting protein); sequestosome 1; Paget disease of bone 3; oxidative stress induced like; autophagy receptor p62
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Quick Database Links
Gene Symbol
SQSTM1
UniProt
UniProt Details
− No UniProt data available
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
FC
Flow Cytometry
IF
Immunofluorescence
ICC
Immunocytochemistry
IP
Immunoprecipitation