SPTBN2 Antibody [SPTBN2/1582]

• Catalog Number: orb1252199
• Category: Antibodies
• Description: SPTBN2 Antibody [SPTBN2/1582]
• Target: SPTBN2
• Clonality: Monoclonal
• Isotype: IgG2a, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: 0.2 mg/mL
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Purification: Protein G affinity chromatography
• Immunogen: Human recombinant partial protein corresponding to amino acids 356-475 was used as the immunogen for this Spectrin beta III antibody.
• UniProt ID: O15020
• Tested applications: ELISA, FC, IF, IHC-P, WB
• Application notes: Flow Cytometry: 0.5-1ug/10^6 cells in 0.1mlIF: 1-2 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 min at RTWB: 0.5-1 ug/mlELISA: 2-4 ug/ml; order the BSA-free formulation for coatingThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Spectrin beta III antibody to be titered up or down for optimal performance.
• Antibody Type: Primary Antibody
• Clone Number: SPTBN2/1582
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Research Area: Neuroscience, Signal Transduction
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human pancreas with Spectrin beta III antibody (clone SPTBN2/1582). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.

Western blot testing of human 1) HeLa and 2) HEK293 cell lysate with Spectrin beta III antibody (clone SPTBN2/1582). Predicted molecular weight ~246 kDa.

SDS-PAGE Analysis of Purified, BSA-Free Spectrin beta III Antibody (clone SPTBN2/1582). Confirmation of Integrity and Purity of the Antibody.