Alpha II Spectrin Antibody / Alpha Fodrin / SPTAN1 / NEAS

SKU: orb2643810

Description

Spectrin, an actin binding protein that is a major component of the cytoskeletal superstructure of the erythrocyte plasma membrane, is essential in determining the properties of the membrane including its shape and deformability. Spectrins function as membrane organizers and stabilizers, composed of nonhomologous II, a neuronal cytoskeleton protein.

Research Area

Neuroscience

Images & Validation

• Tested Applications: IHC-P
• Dilution Range: Immunohistochemistry (FFPE): 1-2ug/ml
• Reactivity: Human
• Application Notes:
  Optimal dilution of the Alpha II Spectrin antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2c, kappa
• Clone No.: SPTAN1/3352
• Immunogen: A recombinant human partial protein (amino acids 2351-2475) was used as the immunogen for this SPTAN1 antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using SPTAN1 antibody (clone SPTAN1/3352). These results demonstrate the foremost specificity of the SPTAN1/3352 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

IHC staining of FFPE human lung with Alpha II Spectrin antibody (clone SPTAN1/3352). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.

IHC staining of FFPE human lung with Alpha II Spectrin antibody (clone SPTAN1/3352). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free Alpha II Spectrin antibody (clone SPTAN1/3352) as confirmation of integrity and purity.

Quick Database Links

UniProt

Q13813