SPRR3 Polyclonal Antibody

• Catalog Number: orb1421224
• Category: Antibodies
• Description: Rabbit polyclonal antibody to SPRR3.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Rat
• UniProt ID: Q9UBC9
• Tested applications: ELISA, ICC, IF, IHC-Fr, IHC-P
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Small proline-rich protein 3, 22 kDa pancornulin,
• Research Area: Epigenetics
• Note: For research use only

Sample: U937 (Human) Cell Lysate at 30 ug. Primary: Anti- SPRR3 at 1/1000 dilution. Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution. Predicted band size: 18 kD. Observed band size: 18 kD.

Paraformaldehyde-fixed, paraffin embedded (Human esophageal cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (SPRR3) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.