SPRR1B Antibody (C-term)

• Catalog Number: orb1928135
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB23641
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, IHC-P: 1:50~100, WB: 1:1000
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 9888 Da
• Target: This SPRR1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 63-89 amino acids from the C-terminal region of human SPRR1B.
• UniProt ID: P22528
• NCBI: NP_003116.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Cornifin-B, 149 kDa pancornulin, Small proline-ric
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of SPRR1B Antibody (C-term) in MDA-MB231, MDA-MB435 cell line lysates (35 ug/lane). SPRR1B (arrow) was detected using the purified Pab.

All lanes: Anti-SPRR1B Antibody (C-term) at 1:1000 dilution + SK-BR-3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/15000 dilution. Observed band size: 10 KDa Blocking/Dilution buffer: 5% NFDM/TBST.

Formalin-fixed and paraffin-embedded human breast carcinoma reacted with SPRR1B Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.