SPRED1 Antibody

SKU: orb1238900

Description

SPRED1 Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  Spred1 antibody can be used for detection of Spred1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Spred1 antibody was raised against a 14 amino acid synthetic peptide near the center of the human Spred1.The immunogen is located within amino acids 250 - 300 of Spred1.
• Target: SPRED1
• Molecular Weight: Predicted: 49 kDa Observed: 498 kDa
• Purification: Spred1 Antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Spred1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• Disclaimer: For research use only

Alternative Names

Spred1 Antibody: NFLS, hSpred1, spred-1, Sprouty-related, EVH1 domain-containing protein 1, Spred-1

Western blot analysis of Spred1 in human brain tissue lysate with Spred1 antibody at (A) 1 and (B) 2 µg/mL.

Immunohistochemistry of Spred1 in human brain tissue with Spred1 antibody at 2.5 µg/mL.

Immunofluorescence of Spred1 in Human Brain tissue with Spred1 antibody at 20 µg/mL.

Quick Database Links

Gene Symbol

SPRED1

UniProt

Q7Z699

RefSeq (Protein):NP_689807