SPRED1 Antibody

• Catalog Number: orb1238900
• Category: Antibodies
• Description: SPRED1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Spred1 antibody was raised against a 14 amino acid synthetic peptide near the center of the human Spred1.The immunogen is located within amino acids 250 - 300 of Spred1.
• Concentration: 1 mg/mL
• Dilution range: Spred1 antibody can be used for detection of Spred1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 49 kDa Observed: 498 kDa
• Target: SPRED1
• UniProt ID: Q7Z699
• NCBI: NP_689807
• Storage: Spred1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Spred1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: Spred1 Antibody: NFLS, hSpred1, spred-1, Sprouty-r
• Note: For research use only
• Application notes: Spred1 antibody can be used for detection of Spred1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Spred1 in human brain tissue lysate with Spred1 antibody at (A) 1 and (B) 2 µg/mL.

Immunohistochemistry of Spred1 in human brain tissue with Spred1 antibody at 2.5 µg/mL.

Immunofluorescence of Spred1 in Human Brain tissue with Spred1 antibody at 20 µg/mL.