SPINT2 Antibody

• Catalog Number: orb1249064
• Category: Antibodies
• Description: SPINT2 Antibody
• Target: SPINT2
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: C-PSAPRRQDSEDH
• UniProt ID: O43291
• Tested applications: ELISA, IHC, WB
• Application notes: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 19kDa band observed in Human Placenta lysates (calculated MW of 21.8kDa according to NP_001159575.1). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Testis. Recommended concentration: 3.75ug/ml.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: SPINT2, serine peptidase inhibitor, Kunitz type, 2
• Research Area: Cancer Biology
• Note: For research use only
• NCBI: NP_066925.1, NP_001159575.1

orb1249064 (0.3 ug/ml) staining of Human Placenta lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1249064 (3.75 ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.