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Catalog Number | orb1248960 |
---|---|
Category | Antibodies |
Description | SPI1 Antibody |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Predicted Reactivity | Rat |
Reactivity | Human |
Immunogen | The immunogen for this antibody is: C-DLYQRQTHEYY |
Concentration | 500 ug/mL |
Dilution range | Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 40kDa band observed in lysates of DAUDI and K562 (calculated MW of 31.2kDa according to NP_001074016.1 and of 31.1kDa according to NP_003111.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Harendza S et al, J Biol Chem. 2000 Jun 30;275(26):19552-9.; PMID: 10867017). Recommended concentration: 0.03-0.1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Spleen and Colon (lymphoid aggregate). Recommended concentration: 3.75ug/ml. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | SPI1 |
UniProt ID | P17947 |
NCBI | NP_001074016.1, NP_003111.2 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | PU.1, SPI1, spleen focus forming virus (SFFV) prov Read more... |
Note | For research use only |
Application notes | Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 40kDa band observed in lysates of DAUDI and K562 (calculated MW of 31.2kDa according to NP_001074016.1 and of 31.1kDa according to NP_003111.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Harendza S et al, J Biol Chem. 2000 Jun 30;275(26):19552-9.; PMID: 10867017). Recommended concentration: 0.03-0.1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Spleen and Colon (lymphoid aggregate). Recommended concentration: 3.75ug/ml. |
Expiration Date | 12 months from date of receipt. |
orb1248960 (0.03 ug/ml) staining of DAUDI lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
orb1248960 (3.75 ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
orb1248960 (3.75 ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
IHC-P, WB | |
Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC-P, WB | |
Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC-P, WB | |
Human, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
ELISA, FC, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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