Spi-C rabbit pAb

• Catalog Number: orb766359
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Spi-C
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Immunogen: The antiserum was produced against synthesized peptide derived from human SPIC. AA range:131-180
• MW: 29
• Tested applications: ELISA, IF, WB
• Dilution range: WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-SPIC antibody, anti-Transcription factor Spi-
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot analysis of various cells using Spi-C Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit.

Western blot analysis of lysates from HepG2 and HeLa cells, using SPIC Antibody. The lane on the right is blocked with the synthesized peptide.

Western blot analysis of the lysates from Jurkat cells using SPIC antibody.

Immunohistochemical analysis of paraffin-embedded human oophoroma. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).