Sphingosine-1-phosphate

SKU: orb1819284

Description

Sphingosine-1-phosphate (S1P) is a bioactive lipid that functions both as an intracellular second messenger and an extracellular agonist for multiple GPCRs, including S1P1-5 and GPR3/6/12. It is widely used in research to study immunology, cancer, and cardiovascular biology in both cellular and animal models.

Research Area

Metabolism Research, Pharmacology & Drug Discovery

Key Properties

• CAS Number: 26993-30-6
• MW: 379.47
• Purity: 98.66%
• Formula: C₁₈H₃₈NO₅P
• SMILES: [C@@H]([C@@H](/C=C/CCCCCCCCCCCCC)O)(COP(=O)(O)O)N
• Target: LPL Receptor,Endogenous Metabolite,S1P Receptor
• Solubility: H2O:< 1 mg/mL (insoluble);NaOH:12.5 mg/mL (32.94 mM)

Bioactivity

• In Vitro:
  Sphingosine-1-phosphate (S1P) (1 μM) induces significant Ca2+ release in HEK293 cells under serum starvation conditions (1% FCS). In functional Ca2+ assays, Suramin alone had no effect on intracellular Ca2+ release via gpr3, gpr6, or gpr12. In contrast, S1P (1 μM) induces Ca2+ release from gpr3, gpr6, and gpr12 in transfected HEK293 cells in the presence of varying concentrations of suramin. In a functional Ca2+ assay, S1P (3-3000 nM) exhibits nanomolar EC50 values in the presence of Suramin (300 μM) for gpr3 (EC50=29 nM), gpr6 (EC50=15 nM) and gpr12 (EC50=24 nM ), rat gpr3 (EC50=68 nM), respectively act on HEK293 cells. S1P increases the expression of S1P1 and Gqi5, and when used at a concentration of 200 nM, it allows Gi proteins to stimulate phospholipase C, as well as TAg-Jurkat cells expressing S1P2 and S1P3 receptors (EC 50s=8 and 11 nM).

Storage & Handling

• Storage: store at low temperature,keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

S1P1, Sphingosine-1-phosphate (d18:1), Endogenous Metabolite, LPL Receptor

Compound Identifiers

PubChem CID

5283560