EDG1 Rabbit Polyclonal Antibody

• Catalog Number: orb100426
• Category: Antibodies
• Description: EDG1 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Canine, Equine
• Reactivity: Human, Mouse, Rat
• Immunogen: KLH conjugated synthetic peptide derived from human EDG1/CD363 (51-150/382aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 44 kDa
• Target: S1PR1
• UniProt ID: P21453
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: CD363; CHEDG1; D1S3362; ECGF1; EDG1; EDG 1; Endoth
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Korbelik, Mladen et al. Ceramide and sphingosine-1-phosphate act as photodynamic therapy-elicited damage-associated molecular patterns: cell surface exposure Int Immunopharmacol, 20, 359-365 (2014)

PubMed: 24713544

Paraformaldehyde-fixed, paraffin embedded (Human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (EDG1) Polyclonal Antibody, Unconjugated (orb100426) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (EDG1) Polyclonal Antibody, Unconjugated (orb100426) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: HUVEC (Human) Cell Lysate at 30 ug, Primary: Anti-EDG1 (orb100426) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 44 kD, Observed band size: 54 kD.

Sample: Lane 1: Mouse Cerebellum tissue lysates, Lane 2: Mouse Cerebrum tissue lysates, Lane 3: Rat Cerebellum tissue lysates, Lane 4: Rat Cerebrum tissue lysates, Primary: Anti-EDG1 (orb100426) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 44 kDa, Observed band size: 50 kDa.