Spectrin alpha chain, non-erythrocytic 1 Antibody

SKU: orb3167421

Description

Our Anti-Spectrin alpha chain, non-erythrocytic 1 mouse monoclonal primary antibody detects bovine, human, mouse, pig, and rat Spectrin alpha chain, non-erythrocytic 1, and is affinity-purified. It is validated for use in FC, ICC, IHC-Frozen, WB.

Images & Validation

• Tested Applications: FC, ICC, IHC, WB
• Dilution Range: WB: 1:5000-1:10000; IHC: 1:1000-1:2000; ICC: 1:1000-1:2000
• Reactivity: Bovine, Human, Mouse, Porcine, Rat
• Application Notes:
  For Flow Cytometry, use ~ 2 µg antibody per ~10^6 cells. Optimal concentrations/dilutions should be determined by the end-user.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: 3D7
• Immunogen: This antibody was raised against a recombinant construct containing the seventh, eight and ninth repeats (amino acids 676-1043) of human alpha-II Spectrin. The 9th spectrin repeat also includes a Src-homology 3 domain. This construct was expressed in and purified from E. coli.
• Target: Spectrin alpha chain, non-erythrocytic 1
• Molecular Weight: 240 kDa
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, and sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

SPTAN1; alpha II spectrin; alpha Fodrin; Spectrin, non-erythroid alpha chain; SPTA2;

Quick Database Links

Gene Symbol

Spectrin alpha chain, non-erythrocytic 1

UniProt

Q13813