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Catalog Number | orb1929895 |
---|---|
Category | Antibodies |
Description | Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB20252 |
Tested applications | FC, IHC-P, WB |
Reactivity | Human, Mouse |
Isotype | Rabbit IgG |
Antibody Type | Primary Antibody |
Dilution range | WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Conjugation | Unconjugated |
MW | 20313 Da |
Target | This SPCS3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 152-180 amino acids from the C-terminal region of human SPCS3. |
UniProt ID | P61009 |
NCBI | NP_068747.1 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Signal peptidase complex subunit 3, 34--, Microsom Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of SPCS3 antibody (C-term) in mouse liver tissue lysates (35 ug/lane). SPCS3 (arrow) was detected using the purified Pab.
SPCS3 Antibody (C-term) flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with SPCS3 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.