SPARC rabbit pAb

• Catalog Number: orb767126
• Category: Antibodies
• Description: Rabbit polyclonal antibody to SPARC
• Clonality: Polyclonal
• Tested applications: ELISA, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from the Internal region of human SPARC. AA range:141-190
• Concentration: 1 mg/ml
• Dilution range: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/20000. Not yet tested in other applications.
• Conjugation: Unconjugated
• MW: 34
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Alternative names: Anti-SPARC antibody, anti-ON antibody, anti-SPARC
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot analysis of HeLa, HepG2 cells using SPARC Polyclonal Antibody. Secondary antibody was diluted at 1:20000.

Immunohistochemical analysis of paraffin-embedded mouse-muscle, antibody was diluted at 1:100.