SPARC / Osteonectin Antibody

SKU: orb1410185

Description

Mouse monoclonal antibody to SPARC

Images & Validation

• Tested Applications: IHC, WB
• Reactivity: Human

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone No.: OSTN/3932
• Immunogen: Recombinant fragment (around aa1-200) of human SPARC protein (exact sequence is proprietary)
• Target: SPARC
• Source/Expression System: Bioreactor
• Molecular Weight: 38kDa
• Purification: Protein A

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Disclaimer: For research use only

Alternative Names

Basement membrane protein 40; BM40; Cysteine rich protein; OI17; ON; Osteonectin; Secreted acidic cystein rich glycoprotein; Secreted protein acidic and cysteine rich; Secreted protein acidic and rich in cysteine (SPARC)

Analysis of Protein Array containing more than 19000 full-length human proteins using SPARC / Osteonectin Mouse Monoclonal Antibody (OSTN/3932). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Formalin-fixed, paraffin-embedded human spleen stained with SPARC / Osteonectin Mouse Monoclonal Antibody (OSTN/3932).

Formalin-fixed, paraffin-embedded human tonsil stained with SPARC / Osteonectin Mouse Monoclonal Antibody (OSTN/3932). Inset: PBS instead of primary antibody; secondary only negative control.

SDS-PAGE Analysis Purified SPARC / Osteonectin Mouse Monoclonal Antibody (OSTN/3932). Confirmation of Purity and Integrity of Antibody.

Western blot analysis of JEG-3 cell lysate using SPARC / Osteonectin Mouse Monoclonal Antibody (OSTN/3932).

Quick Database Links

Gene Symbol

SPARC

UniProt

P09486