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Catalog Number | orb1258275 |
---|---|
Category | Antibodies |
Description | SPAM1 Antibody |
Target | SPAM1 |
Clonality | Polyclonal |
Isotype | IgG |
Conjugation | Unconjugated |
Reactivity | Human, Mouse, Rat |
Form/Appearance | Liquid |
Concentration | batch dependent |
Buffer/Preservatives | Liquid |
Purification | Affinity purification |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 100-300 of human SPAM1 (NP_003108.2). |
UniProt ID | P38567 |
MW | Observed: 47kDa |
Tested applications | IF, WB |
Application notes | WB: 1:500 - 1:2000IF: 1:50 - 1:200 |
Antibody Type | Primary Antibody |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | HEL-S-96n, HYA1, HYAL1, HYAL3, HYAL5, PH-2PH2SPAG1 Read more... |
Note | For research use only |
Western blot analysis of extracts of various cell lines, using SPAM1 antibody (orb1258275) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence analysis of HeLa cells using SPAM1 antibody (orb1258275). Blue: DAPI for nuclear staining.
FC, IF, IHC-Fr, IHC-P | |
Mouse, Rat | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, IH, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |