SPAM1 Antibody

• Catalog Number: orb1258275
• Category: Antibodies
• Description: SPAM1 Antibody
• Target: SPAM1
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Liquid
• Purification: Affinity purification
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 100-300 of human SPAM1 (NP_003108.2).
• UniProt ID: P38567
• MW: Observed: 47kDa
• Tested applications: IF, WB
• Application notes: WB: 1:500 - 1:2000IF: 1:50 - 1:200
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: HEL-S-96n, HYA1, HYAL1, HYAL3, HYAL5, PH-2PH2SPAG1
• Research Area: Cancer Biology, Cell Biology, Signal Transduction
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using SPAM1 antibody (orb1258275) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunofluorescence analysis of HeLa cells using SPAM1 antibody (orb1258275). Blue: DAPI for nuclear staining.