SP140 Antibody (Center)

• Catalog Number: orb1937117
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Target: This SP140 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 272-301 amino acids from the Central region of human SP140.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: Q13342
• MW: 98223 Da
• Tested applications: IF, IHC-P, WB
• Dilution range: IF: 1:10~50, WB: 1:1000, IHC-P: 1:10~50
• Antibody Type: Primary Antibody
• Clone Number: RB32702
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Nuclear body protein SP140 {ECO:0000312|HGNC:HGNC:
• Research Area: Immunology & Inflammation
• Note: For research use only
• NCBI: NP_001005176.1, NP_001265380.1, NP_001265382.1, NP_001265381.1, NP_009168.4

Fluorescent confocal image of Hela cell stained with SP140 Antibody (Center). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SP140 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). SP140 immunoreactivity is localized to Cytoplasm and Nucleus significantly.

SP140 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of SP140 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

SP140 Antibody (Center) western blot analysis in HL-60 cell line lysates (35 ug/lane). This demonstrates the SP140 antibody detected the SP140 protein (arrow).