SP100 Antibody

• Catalog Number: orb1410458
• Category: Antibodies
• Description: Mouse monoclonal antibody to SP100
• Target: SP100
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG2b
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Concentration: Purified Ab with BSA and Azide at 200ug/ml
• Buffer/Preservatives: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Immunogen: Recombinant full-length human SP100 protein
• UniProt ID: P23497
• MW: 100.4kDa
• Tested applications: ELISA, FC, IF, IHC, WB
• Dilution range: ELISA (For coating, order antibody without BSA); Western Blot (1-2ug/ml); Immunofluorescence (0.5-2ug/ml);,Immunoprecipitation (1-2ug per 100-500ug of total protein (1ml of cell lysate)); Flow Cytometry (0.5-2ug/million cells); ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
• Application notes: Positive Control: HeLa or U87 cells
• Clone Number: PCRP-SP100-1B9
• Expression System: Bioreactor
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Lysp100b; Nuclear antigen Sp100; Nuclear autoantig
• Note: For research use only
• Entrez: 6672