SP100 Antibody

SKU: orb1410458

Description

Mouse monoclonal antibody to SP100

Images & Validation

• Tested Applications: ELISA, FC, IF, IHC, WB
• Reactivity: Human

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone No.: PCRP-SP100-1B9
• Immunogen: Recombinant full-length human SP100 protein
• Target: SP100
• Source/Expression System: Bioreactor
• Molecular Weight: 100.4kDa
• Purification: Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Disclaimer: For research use only

Alternative Names

Lysp100b; Nuclear antigen Sp100; Nuclear autoantigen Sp 100; Nuclear dot associated Sp100 protein; SP100; SP100 HMG nuclear autoantigen; SP100 nuclear antigen; Speckled 100 kDa

Analysis of Protein Array containing more than 19000 full-length human proteins using SP100 Mouse Monoclonal Antibody (PCRP-SP100-1B9). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Flow cytometric analysis of PFA-fixed HeLa cells. SP100 Mouse Monoclonal Antibody (PCRP-SP100-1B9) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).

IHC analysis of formalin-fixed, paraffin-embedded human colon. Strong nuclear staining using PCRP-SP100-1B9 at 2 ug/ml in PBS for 30 min RT. HIER: Tris/EDTA, pH9.0, 45 min. 2°: HRP-polymer, 30 min. DAB, 5 min.

IHC analysis of formalin-fixed, paraffin-embedded human tonsil. Strong nuclear staining using PCRP-SP100-1B9 at 2 ug/ml in PBS for 30 min RT. HIER: Tris/EDTA, pH9.0, 45 min. 2°: HRP-polymer, 30 min. DAB, 5 min.

Immunofluorescence Analysis of PFA-fixed HeLa cells stained using SP100 Mouse Monoclonal Antibody (PCRP-SP100-1B9) followed by goat anti-mouse IgG-CF488. Membrane stained with phalloidin.

SDS-PAGE Analysis Purified SP100 Mouse Monoclonal Antibody (PCRP-SP100-1B9). Confirmation of Purity and Integrity of Antibody.

Quick Database Links

Gene Symbol

SP100

UniProt

P23497