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Item 1 of 4
Item 1 of 4
SOX9 Antibody (N-term)
Catalog Number: orb1936138
| Catalog Number | orb1936138 |
|---|---|
| Category | Antibodies |
| Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB13373 |
| Tested applications | IF, IHC-P, WB |
| Predicted Reactivity | Mouse, Porcine, Rat |
| Reactivity | Human |
| Isotype | Rabbit IgG |
| Antibody Type | Primary Antibody |
| Dilution range | IF: 1:10~50, WB: 1:1000, WB: 1:1000, IHC-P: 1:10~50 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Conjugation | Unconjugated |
| MW | 56137 Da |
| Target | This SOX9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 41-70 amino acids from the N-terminal region of human SOX9. |
| UniProt ID | P48436 |
| NCBI | NP_000337.1 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Transcription factor SOX-9, SOX9 Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Western blot analysis of anti-SOX9 Antibody (N-term) in K562 cell line lysates (35 ug/lane). SOX9 (arrow) was detected using the purified Pab.
Western blot analysis of SOX9 (arrow) using rabbit polyclonal SOX9 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SOX9 gene (Lane 2).
Immunofluorescence analysis of anti-SOX9 Antibody (N-term) in HeLa cells. 0.025 mg/ml primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.
Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with SOX9 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
