SOX2 Antibody (N-term)

SKU: orb1931757

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: FC, IF, IHC-P, WB
• Dilution Range: IF - 1:10-50, WB - 1:1000, IHC-P - 1:10-50, FC - 1:10-50
• Reactivity: Human
• Predicted Reactivity: Mouse, Other, Sheep, Zebrafish

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB14026
• Immunogen: This SOX2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89-119 amino acids from the N-terminal region of human SOX2. Antigen Region: 89-119 aa.
• Target: SOX2
• Molecular Weight: 34310 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Transcription factor SOX-2, SOX2

Western blot analysis of SOX2 Antibody (N-term) in 293 cell line lysates (35 ug/lane). SOX2 (arrow) was detected using the purified Pab.

Western blot analysis of SOX2 (arrow) using rabbit polyclonal SOX2 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SOX2 gene (Lane 2).

Flow cytometric analysis of NCI-H292 cells using SOX2 Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunofluorescence analysis of SOX2 Antibody (N-term) in HeLa cells. 0.025 mg/ml primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with SOX2 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Quick Database Links

Gene Symbol

SOX2

UniProt

P48431

RefSeq (Protein):NP_003097.1