Sox-9 (phospho Ser181) rabbit pAb

SKU: orb770129

Description

Rabbit polyclonal antibody to Sox-9 (phospho-Ser181)

Images & Validation

• Tested Applications: ELISA, IF, IHC-P, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
• Reactivity: Human, Mouse

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human SOX-9 around the phosphorylation site of Ser181. AA range:147-196
• Molecular Weight: 56
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Anti-SOX9 antibody, anti-Transcription factor SOX-9 antibody

Western blot analysis of Sox-9 (phospho Ser181) Polyclonal Antibody, using Hela, HepG2 cell treated or untreated with UV, 4°C over night, secondary antibody was diluted at 1:10000, 37°C 1hour.

Immunofluorescence analysis of A549 cells, using SOX-9 (Phospho-Ser181) Antibody. The picture on the right is blocked with the phospho peptide.

Immunohistochemistry analysis of paraffin-embedded human brain, using SOX-9 (Phospho-Ser181) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from 293 cells treated with PBS 60', using SOX-9 (Phospho-Ser181) Antibody. The lane on the right is blocked with the phospho peptide.