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Sox-9 (phospho Ser181) rabbit pAb

Catalog Number: orb770129

DispatchUsually dispatched within 5-10 working days
$ 250.00
Catalog Numberorb770129
CategoryAntibodies
DescriptionRabbit polyclonal antibody to Sox-9 (phospho-Ser181)
ClonalityPolyclonal
Tested applicationsELISA, IF, IHC-P, WB
ReactivityHuman, Mouse
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from human SOX-9 around the phosphorylation site of Ser181. AA range:147-196
Concentration1 mg/ml
Dilution rangeWestern Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
ConjugationUnconjugated
MW56
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesPBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Alternative namesAnti-SOX9 antibody, anti-Transcription factor SOX-
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NoteFor research use only
Expiration Date12 months from date of receipt.
Sox-9 (phospho Ser181) rabbit pAb

Western blot analysis of Sox-9 (phospho Ser181) Polyclonal Antibody, using Hela, HepG2 cell treated or untreated with UV, 4°C over night, secondary antibody was diluted at 1:10000, 37°C 1hour.

Sox-9 (phospho Ser181) rabbit pAb

Immunofluorescence analysis of A549 cells, using SOX-9 (Phospho-Ser181) Antibody. The picture on the right is blocked with the phospho peptide.

Sox-9 (phospho Ser181) rabbit pAb

Immunohistochemistry analysis of paraffin-embedded human brain, using SOX-9 (Phospho-Ser181) Antibody. The picture on the right is blocked with the phospho peptide.

Sox-9 (phospho Ser181) rabbit pAb

Western blot analysis of lysates from 293 cells treated with PBS 60', using SOX-9 (Phospho-Ser181) Antibody. The lane on the right is blocked with the phospho peptide.