Sodium Potassium ATPase alpha-1 (Phospho-Tyr260) Rabbit pAb Antibody

SKU: orb771491

Description

Rabbit polyclonal antibody to Sodium Potassium ATPase alpha-1 (Phospho-Tyr260)

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
• Reactivity: Human, Mouse, Rat

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Synthetic peptide from human protein at AA range: 230-290
• Molecular Weight: 115
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-Sodium/potassium-transporting ATPase subunit alpha-1 (Na(+)/K(+) ATPase alpha-1 subunit) (EC 3.6.3.9) (Sodium pump subunit alpha-1) antibody

Western blot analysis of KB Hela lysate, antibody was diluted at 1000. Secondary antibody was diluted at 1:20000.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Na + /K + -ATPase α1 (Phospho-Tyr260) Antibody.

Western blot analysis of lysates from 293 cells treated with PMA, using phospho-Na + /K + -ATPase α1 (Phospho-Tyr260) antibody.

Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Quick Database Links

UniProt

P05023