Sodium-Iodide Symporter Antibody: FITC

• Catalog Number: orb149658
• Category: Antibodies
• Description: Mouse monoclonal antibody against NIS conjugated to FITC
• Target: Sodium Iodide Symporter
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG1
• Conjugation: FITC
• Reactivity: Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: 640.91mM DMSO, 136.36mM Ethanolamine, 9.09mM Sodium Bicarbonate in 90.9% PBS
• Purification: Protein G Purified
• Immunogen: Mannose binding protein hNIS fusion (AA468-643)
• UniProt ID: Q92911
• MW: 15kDa
• Tested applications: ICC, IF, IHC
• Dilution range: WB (1:1000), IHC (1:1000)
• Application notes: 1 µg/ml of SMC-390 was sufficient for detection of hNIS in 20 µg of transfected COS-7 cell membrane lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Clone Number: 14F
• Storage: Conjugated antibodies should be stored according to the product label
• Alternative names: NIS antibody, SLC5A5 antibody, solute carrier fami
• Research Area: Cancer Biology, Neuroscience, Pharmacology & Drug
• Note: For research use only
• Entrez: 6528
• NCBI: NP_000444.1

Immunohistochemistry analysis using Mouse Anti-Sodium Iodide Symporter Monoclonal Antibody, Clone 14F. Tissue: Thyroid. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-Sodium Iodide Symporter Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 μl for 5 minutes at RT.

Western Blot analysis of Human thyroid lysate showing detection of Sodium Iodide Symporter protein using Mouse Anti-Sodium Iodide Symporter Monoclonal Antibody, Clone 14F. Primary Antibody: Mouse Anti-Sodium Iodide Symporter Monoclonal Antibody at 1:1000.

Immunohistochemistry analysis using Mouse Anti-Sodium Iodide Symporter Monoclonal Antibody, Clone 14F. Tissue: Trachea. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-Sodium Iodide Symporter Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 μl for 5 minutes at RT.

Immunohistochemistry analysis using Mouse Anti-Sodium Iodide Symporter Monoclonal Antibody, Clone 14F. Tissue: Thyroid. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-Sodium Iodide Symporter Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 μl for 5 minutes at RT.

Immunohistochemistry analysis using Mouse Anti-Sodium Iodide Symporter Monoclonal Antibody, Clone 14F. Tissue: Thyroid. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-Sodium Iodide Symporter Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 μl for 5 minutes at RT.