SOD1 Recombinant Rabbit Monoclonal Antibody

• Catalog Number: orb1816706
• Category: Antibodies
• Description: SOD1 Recombinant Rabbit Monoclonal Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: 10B4
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Mouse, Rat
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human SOD1
• Antibody Type: Recombinant Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-1000, IHC-P=1:100-500, IF=1:50-100, IHC-F=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 17 kDa
• Target: SOD1
• UniProt ID: P00441
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS, Glycerol, rAlbumin.
• Alternative names: Superoxide Dismutase 1; ALS 1; ALS; ALS1; Amyotrop
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (human colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (SOD1) Monoclonal Antibody, Unconjugated (orb1816706) at 1:300 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (SOD1) Monoclonal Antibody, Unconjugated (orb1816706) at 1:300 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (SOD1) Monoclonal Antibody, Unconjugated (orb1816706) at 1:300 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (SOD1) Monoclonal Antibody, Unconjugated (orb1816706) at 1:300 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (SOD1) Monoclonal Antibody, Unconjugated (orb1816706) at 1:300 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Mouse Cerebrum tissue lysates, Lane 2: Mouse Liver tissue lysates, Lane 3: Mouse Placenta tissue lysates, Lane 4: Rat Cerebrum tissue lysates, Lane 5: Rat Placenta tissue lysates, Lane 6: Human HepG2 cell lysates, Lane 7: Human HeLa cell lysates, Lane 8: Human Jurkat cell lysates, Lane 9: Human MCF-7 cell lysates, Primary: Anti-SOD1 (orb1816706) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 17 kDa, Observed band size: 15 kDa.