SOD1 (EDI) Antibody (PerCP)

SKU: orb152276

Description

Rabbit polyclonal to SOD1 (PerCP). Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body. It works by catalyzing the dismutation of the superoxide radical O2 to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (1,4). In general, SODs play a major role in antioxidant defense mechanisms. There are two main types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge. Misfolding of SOD1 has been implicated in Amyotrophic lateral sclerosis (ALS). Therefore conformation specific antibodies such as SOD1 (EDI), which targets an exposed region of the dimer interface (EDI) of SOD1, are useful for determining the conformation of SOD1 in affected tissues. This antibody can be used in conjunction with SOD1 (U beta B) which detects an unfolded beta barrel (U beta B) of SOD1..

Research Area

Metabolism Research

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: WB (1:1000)
• Reactivity: Human, Mouse, Porcine, Rat
• Application Notes:
  1µg/ml was sufficient for detection of the exposed dimer interface of SOD1 by colorimetric dot blot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: C-terminal region of SOD1, exposed dimer interface (EDI)
• Target: SOD1 (EDI)
• Purification: Protein A Purified
• Conjugation: PerCP

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 95.46mM Phosphate, 2.48mM MES and 2mM EDTA
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

SOD1, Superoxide dismutase [Cu-Zn], Cu/Zn superoxide dismutase, SODC_HUMAN, ALS1, SOD1 EDI, SEDI, SOD1 exposed dimer interface, Conformationally altered SOD1, Misfolded SOD1, Apo-SOD1, Superoxide dismutase 1, SOD

Quick Database Links

Gene Symbol

SOD1 (EDI)

UniProt

P00441

NCBI Gene ID

6647

RefSeq:CAG46542