SOD1 Antibody (Center)

SKU: orb1928316

Description

Research Area

Cancer Biology, Cell Biology, Metabolism Research, Neuroscience, Signal Transduction

Images & Validation

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Item 1 of 4

Tested Applications	FC, IF, IHC-P, WB
Dilution Range	WB - 1:1000, IHC-P - 1:100-500, FC - 1:10-50, IF - 1:10-50
Reactivity	Human

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Molecular Weight	15936 Da
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Form/Appearance	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Quality Guarantee

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Western blot analysis of SOD1 Antibody (Center) in Jurkat cell line lysates (35 ug/lane). SOD1 (arrow) was detected using the purified Pab.

Confocal immunofluorescent analysis of SOD1 Antibody (Center) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).

SOD1 Antibody (Center) flow cytometric analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human colon carcinoma reacted with SOD1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.