SNX26 Antibody

SKU: orb1246931

Description

SNX26 Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, IHC
• Reactivity: Human
• Predicted Reactivity: Mouse
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Preliminary experiments gave an approx 75kDa band in Human Bone Marrow, Kidney, and Pancreas lysates after 1µg/mL antibody staining. EB05916 may detect the isoform A1A5D2 (UniProtKB) with a molecular weight of 81.4kDa which is similar in amino acid sequence to NP_443180.2 however lacking aa729-1268. Please note that currently we cannot find a precise explanation in the literature for the band we observe given the calculated size of 121kDa according to NP_443180.2. The 75kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Brain shows cytoplasm staining of Purkinje cells in Cerebellum. Recommended concentration, 2.5µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: SWSLHSEGQTRSYC
• Target: SNX26
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

SNX26, TCGAP, FLJ39019, sorting nexin 26, likely ortholog of mouse TC10/CDC42 GTPase-activating protein, NOMA-GAP, neurite outgrowth multiadaptor RhoGAP protein

orb1246931 (2.5 ug/ml) staining of paraffin embedded Human Brain. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

SNX26

UniProt

O14559

RefSeq (Protein):NP_443180.2